DFID ANIMAL HEALTH PROGRAMME PROJECT R7363 FINAL TECHNICAL REPORT Investigation of the immunogenic potential of heartwater (Cowdria ruminantium) grown in tick cell lines
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ruminantium) grown in tick cell lines
• Heartwater can cause high mortality amongst cattle, sheep and goats. It is caused by the tick-borne organism Cowdria ruminantium. • The disease has a major impact upon the development of livestock owning communities especially those in small-holder dairy schemes. • Existing vaccines against heartwater, a tickborne disease which is a major constraint to small ruminant production in sub-Saharan...
متن کاملIdentification of drugs effective against Cowdria ruminantium using a mouse screening system.
Heartwater is a tick-transmitted rickettsial infection of ruminants, caused by Cowdria ruminantium. The tetracyclines are the only compounds available for therapy of the disease. A screen, using mice infected with C. ruminantium, was developed and used to identify new compounds with potential for the control of heartwater. A series of di-4-methyl-thiosemicarbazones was shown to be highly active...
متن کاملGrowth of Cowdria ruminantium, the causative agent of heartwater, in a tick cell line.
The tick-borne rickettsia Cowdria ruminantium has been propagated continuously for over 500 days in the Ixodes scapularis tick cell line IDE8 by using the Gardel isolate from bovine endothelial cells as an inoculum. Infection of the tick cells was confirmed by PCR, karyotyping, electron microscopy, and reinfection of bovine cells.
متن کاملProblems with the interpretation of epidemiological data in heartwater: a study on 23 farms.
In an epidemiological study undertaken on 23 farms where heartwater occurs endemically, it was found that on an overall average, antibodies to Cowdria ruminantium were detected in 64.3% of the cattle, 6 adult Amblyomma hebraeum ticks were counted per animal and 7.0% of ticks were infected with the heartwater agent. It was found that the seropositivity of the animals was determined largely by th...
متن کاملHeartwater. The artificial transmission of Cowdria ruminantium in domestic ruminants and mice.
The artificial transmission of Cowdria ruminantium with infected blood, organ homogenates, peritoneal macrophages, tick stabilate and tissue culture cells is discussed. Organ homogenates prepared from the myocardium, spleen, kidneys and liver of diseased animals are commonly used to infect mice. The efficacy of organ homogenates as a source of C. ruminantium depends on factors such as the route...
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